PGTB

The PGTB develops and offers services using medium- and high-throughput technologies based on sequencing (sequencing of short and long DNA fragments, targeted metagenomics, sequencing of genomes and transcriptomes, total metagenomics and metatranscriptomics, gene capture, etc.), mutation search and genotyping (SNP, INDEL and microsatellites), quantification of gene expression, and analysis of environmental, sensitive or ancient DNA in a confined laboratory.

The PGTB is part of the INRAE Genomics Research Infrastructure, with the GeT platform in Toulouse, the Gentyane platform in Clermont-Ferrand and the EPGV platform in Evry. The PGTB is also a member of the France Genomics network, which groups the main genomics platforms at national level.

Expertises :

• Development and genotyping of microsatellite markers using Illumina sequencing
• Targeted metagenomics (metabarcoding) on Illumina and Oxford Nanopore Technologies sequencers
• Metagenomics and metatranscriptomics on Illumina NextSeq 2000 sequencer
• DNAseq and RNAseq on Illumina MiSeq and NextSeq 2000 sequencers
• Agilent SureSelect capture on DNA and methylated DNA
• Oxford Nanopore Technologies long-read sequencing on MinION and P2 solo
• SNP / INDEL genotyping by mass spectrometry on MassArray System (Agena Biosciences)
• Ready-to-load sequencing on NextSeq 2000 of pools of RNAseq, single-cell, ATAC-seq, Chip-seq, WGS libraries, etc.